Device

Part:BBa_M39022:Design

Designed by: Edgar Aranda-Michel   Group: MIT BE 20.20-S12   (2012-05-13)


Kill Switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 63
    Illegal XbaI site found at 623
    Illegal PstI site found at 696
    Illegal PstI site found at 1229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 696
    Illegal PstI site found at 1229
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 63
    Illegal XbaI site found at 623
    Illegal PstI site found at 696
    Illegal PstI site found at 1229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 63
    Illegal XbaI site found at 623
    Illegal PstI site found at 696
    Illegal PstI site found at 1229
    Illegal AgeI site found at 376
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750
    Illegal BsaI.rc site found at 1241
    Illegal SapI site found at 1401


Design Notes

we needed to have a way to make sure both the membrane and genome of the cell was destroyed. These enzymes seemed like the best idea.


Source

e.coli

References